R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TNFRSF17/BCMA (E6D7B) Rabbit mAb #88183
Filter:
- WB
- IP
- IHC
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18-30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:8000 |
| Immunoprecipitation | 1:800 |
| IHC Leica Bond | 1:150 - 1:600 |
| Immunohistochemistry (Paraffin) | 1:300 - 1:1200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #84031.
For a carrier-free (BSA and azide free) version of this product see product #84031.
Protocol
Specificity / Sensitivity
TNFRSF17/BCMA (E6D7B) Rabbit mAb recognizes endogenous levels of total TNFRSF17/BCMA protein. This antibody displays some non-specific staining of peripheral and CNS tissue when used for IHC-P.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu115 of human TNFRSF17/BCMA protein.
Background
B cell maturation antigen (BCMA/TNFRSF17/CD269) is a transmembrane glycoprotein and member of the TNFR superfamily (1). BCMA expression is largely restricted to the B cell lineage. Pro-survival signaling through this receptor plays a pivotal role in humoral immunity by regulating B cell maturation and plasma cell differentiation upon binding its ligands, BAFF and APRIL (2-6). BCMA is expressed in a number of B cell malignancies and has garnered much attention as a novel therapeutic target for the treatment of multiple myeloma due to its selective and elevated expression on the cell surface of malignant plasma cells (7-10).
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- Chiu, A. et al. (2007) Blood 109, 729-39.
- Avery, D.T. et al. (2003) J Clin Invest 112, 286-97.
- O'Connor, B.P. et al. (2004) J Exp Med 199, 91-8.
- Darce, J.R. et al. (2007) J Immunol 178, 5612-22.
- Xu, S. and Lam, K.P. (2001) Mol Cell Biol 21, 4067-74.
- Carpenter, R.O. et al. (2013) Clin Cancer Res 19, 2048-60.
- Tai, Y.T. et al. (2006) Cancer Res 66, 6675-82.
- Moreaux, J. et al. (2004) Blood 103, 3148-57.
- Tai, Y.T. et al. (2014) Blood 123, 3128-38.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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