R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TNF-R2 (E8D7P) Rabbit mAb #72337
Filter:
- WB
- IP
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TNF-R2 (E8D7P) Rabbit mAb recognizes endogenous levels of total TNF-R2 protein. This antibody cross-reacts with an unidentified protein of 180 kDa in some cell extracts.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TNF-R2 protein.
Background
TNF-α is an important cytokine produced by numerous cell types, including neutrophils, activated lymphocytes, macrophages, and NK cells. It plays a critical role in inflammatory responses and apoptosis (1). TNF-α exists as a membrane-anchored and soluble form, both of which show biological activity. Response to TNF-α is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2). Antagonists to TNF-α have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3).
The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa), can mediate distinct cellular responses (4,5). In most cases, cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family, leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12).
The two receptors for TNF-α, TNF-R1 (55 kDa) and TNF-R2 (75 kDa), can mediate distinct cellular responses (4,5). In most cases, cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7). In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8). TNF-R2 binds to distinct members of the TRAF family, leading to the activation of NF-κB (9,10). Soluble forms of both receptors have also been characterized which can bind TNF-α and may play an important role in immune disorders (11,12).
- Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
- Locksley, R.M. et al. (2001) Cell 104, 487-501.
- Taylor, P.C. et al. (2004) Curr Opin Biotechnol 15, 557-63.
- Tartaglia, L.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9292-6.
- Peschon, J.J. et al. (1998) J Immunol 160, 943-52.
- Tartaglia, L.A. et al. (1993) Cell 73, 213-6.
- Rothe, J. et al. (1993) Nature 364, 798-802.
- Aspalter, R.M. et al. (2003) J Leukoc Biol 74, 572-82.
- Rothe, M. et al. (1994) Cell 78, 681-92.
- Rothe, M. et al. (1995) Science 269, 1424-7.
- Humbert, M. et al. (1994) Am J Respir Crit Care Med 149, 1681-5.
- Schröder, J. et al. (1995) Infection 23, 143-8.
限制使用
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