TNF-α (D2D4) XP^® Rabbit mAb (BSA and Azide Free) #57485

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	17,25,28
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

This product is the carrier free version of product #11948. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #11948

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TNF-α (D2D4) XP^® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total TNF-α protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse TNF-α protein.

Background

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
Mouse and rat TNF-α are predicted to be glycosylated, while human TNF-α is not (4).

Database Links

UniProt ID: P06804

Entrez-Gene Id: 21926

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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TNF-α (D2D4) XP® Rabbit mAb (BSA and Azide Free) #57485