R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TMEM41B (F6F4Z) Rabbit mAb #30518
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TMEM41B (F6F4Z) Rabbit mAb recognizes endogenous levels of total TMEM41B protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM41B protein.
Background
TMEM41B (also known as Stasimon) was discovered as a target of splicing dysfunction induced by loss of spinal motor neuron (SMN) protein, a feature of spinal muscular atrophy (SMA), resulting in motor circuit defects (1). Loss of TMEM41B in mice results in embryonic lethality (2). TMEM41B is a transmembrane protein that resides at the endoplasmic reticulum (ER) and is found at contact sites between the ER and mitochondria (2). Independent CRISPR screens identified TMEM41B as a novel regulator of autophagy, being structurally and functionally related to VMP1 (3-5). Loss of TMEM41B inhibited autophagosome formation and autophagic flux (3-5). Furthermore, depletion of TMEM41B led to an accumulation of lipid droplets, indicating a role in lipid mobilization and utilization of fatty acids (3,4). TMEM41B also appeared in screens, identifying it as an essential host protein for infection of flaviviruses and coronaviruses, including SARS-CoV-2 (6,7).
- Lotti, F. et al. (2012) Cell 151, 440-54.
- Van Alstyne, M. et al. (2018) Biochem Biophys Res Commun 506, 463-470.
- Moretti, F. et al. (2018) EMBO Rep19, e45889. doi: 10.15252/embr.201845889.
- Morita, K. et al. (2018) J Cell Biol 217, 3817-3828.
- Shoemaker, C.J. et al. (2019) PLoS Biol 17, e2007044.
- Hoffmann, H.H. et al. (2020) bioRxiv, 2020.10.09.334128. doi: 10.1101/2020.10.09.334128.
- Schneider, W.M. et al. (2020) bioRxiv, 2020.10.07.326462. doi: 10.1101/2020.10.07.326462.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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