R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TMEM175 (F5G5U) Rabbit mAb #56936
Filter:
- WB
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TMEM175 (F5G5U) Rabbit mAb recognizes endogenous levels of total TMEM175 protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse TMEM175 protein.
Background
Transmembrane protein 175 (TMEM175) is a potassium channel located in the membrane of late endosomes and lysosomes. TMEM175 has been shown to regulate lysosomal membrane potential, pH stability, and organelle fusion. In contrast, TMEM175 deficiency impairs lysosomal pH stability, lysosome-mediated autophagosomal clearance, and mitochondrial clearance in cells (1). Dysfunctional mitophagy resulting from TMEM175 loss-of-function mutations has been implicated in several human central nervous system (CNS) diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD) (2-4). Recently, it has been discovered that TMEM175 plays a role in apoptosis, as it is directly inhibited through binding of the anti-apoptotic regulator Bcl-2 (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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