R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TIPE2 (E5Q2S) Rabbit mAb #53842
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:400 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TIPE2 (E5Q2S) Rabbit mAb recognizes endogenous levels of total TIPE2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human TIPE2 protein, including the carboxy terminus.
Background
Tumor necrosis factor-α (TNF-α)-induced protein 8-like 2 (TIPE2) is a negative regulator of innate and adaptive immunity, and is important in maintaining immune homeostasis. TIPE2 inhibits TGF-β activated kinase 1 (TAK1), a key regulator of inflammatory signaling (1). TIPE2 inhibits oncogenic Ras activity, and deficiency of TIPE2 is associated with resistance to cell death and increased cell migration (2). TIPE2 is downregulated in several human cancers, and may serve as a tumor suppressor and biomarker (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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