渲染靶标:SSR
Render Timestamp:
4/4/2025, 7:35:46 AM EDT
4/4/2025, 11:35:46 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:18:22.194
Product last modified at: 2025-03-04T19:30:16.137Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TIM23 (E1Q7L) Rabbit mAb #34822

Filter:
  • WB
  • IF
Western Blotting Image 1: TIM23 (E1Q7L) Rabbit mAb
Western blot analysis of extracts from various cell lines using TIM23 (E1Q7L) Rabbit mAb.

To Purchase # 34822

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 22
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TIM23 (E1Q7L) Rabbit mAb recognizes endogenous levels of total TIM23 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala29 of human TIM23 protein.

Background

TIM23 is a subunit of the mitochondrial import inner membrane translocase complex. This complex mediates translocation of presequence-containing proteins into the mitochondrial matrix and inner membrane. Besides TIM23, there are multiple subunits in the complex, including TIM17A, TIM17B, and TIM50 (1,2). Studies show that mutant huntingtin (mHTT), the protein causing neurodegenerative Huntington’s disease, binds to TIM23 subunit. There are less soluble matrix mitochondrial proteins imported by the TIM23 complex in cells expressing mHTT and in the brain tissues of Huntington’s disease patients when compared with controls (2). In addition, asbestos causes translocation of NOX4 to the mitochondrial matrix through direct interaction with TIM23 in lung macrophages. TIM23 increases NOX4-induced reactive oxygen species (ROS) in mitochondria and metabolic reprogramming to oxidative phosphorylation (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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