R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TIFA (E9W1L) Rabbit mAb #54939
Filter:
- WB
- IP
Western blot analysis of extracts from various cell lines using TIFA (E9W1L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal in the TIFA knockout 293T cells confirms specificity of the antibody for TIFA.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TIFA (E9W1L) Rabbit mAb recognizes endogenous levels of total TIFA protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TIFA protein.
Background
TRAF-interacting protein with FHA domain-containing protein A (TIFA) is a cytosolic adaptor protein that is a critical node in an innate immune signaling axis that is activated upon detection of proinflammatory stimuli derived from a bacterial pathogen-associated molecular pattern metabolite, ADP-heptose (1-4). ADP-heptose binds and activates Alpha-protein kinase 1, which drives the phosphorylation-dependent oligomerization and activation of TIFA (5,6). Activated TIFA is then poised to shape the innate immune response by triggering TRAF6 oligomerization, IKK activation, and NF-κB-dependent proinflammatory gene transcription (1,2,5,7).
- Zhou, P. et al. (2018) Nature 561, 122-6.
- Milivojevic, M. et al. (2017) PLoS Pathog 13, e1006224.
- Zimmermann, S. et al. (2017) Cell Rep 20, 2384-95.
- Gaudet, R.G. et al. (2015) Science 348, 1251-5.
- Huang, C.C. et al. (2012) Mol Cell Biol 32, 2664-73.
- Weng, J.H. et al. (2015) Biochemistry 54, 6219-29.
- Ea, C.K. et al. (2004) Proc Natl Acad Sci U S A 101, 15318-23.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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