R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TIF1α/TRIM24 (E9T3N) Rabbit mAb #79030
Filter:
- WB
- IP
- IHC
Supporting Data
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 140, 160, 180 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TIF1α/TRIM24 (E9T3N) Rabbit mAb recognizes endogenous levels of total TIF1α/TRIM24 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein.
Background
TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17).
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- Lv, D. et al. (2017) Nat Commun 8, 1454.
- Zhu, Y. et al. (2018) Exp Cell Res 367, 274-281.
限制使用
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