Render Target: SSR
Render Timestamp: 2025-01-09T21:48:07.855Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:58:13.175
Product last modified at: 2025-01-01T09:02:21.955Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TIF1α/TRIM24 (E9T3N) Rabbit mAb #79030

Filter:
  • WB
  • IP
  • IHC
Western Blotting Image 1: TIF1α/TRIM24 (E9T3N) Rabbit mAb
Western blot analysis of extracts from various cell lines using TIF1α/TRIM24 (E9T3N) Rabbit mAb.

To Purchase # 79030T

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 140, 160, 180
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TIF1α/TRIM24 (E9T3N) Rabbit mAb recognizes endogenous levels of total TIF1α/TRIM24 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein.

Background

TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17).
  1. Le Douarin, B. et al. (1995) EMBO J 14, 2020-33.
  2. Le Douarin, B. et al. (1996) EMBO J 15, 6701-15.
  3. Heery, D.M. et al. (1997) Nature 387, 733-6.
  4. Le Douarin, B. et al. (1998) J Steroid Biochem Mol Biol 65, 43-50.
  5. Khetchoumian, K. et al. (2008) Cell Cycle 7, 3647-52.
  6. Ignat, M. et al. (2008) Proc Natl Acad Sci U S A 105, 2598-603.
  7. Khetchoumian, K. et al. (2007) Nat Genet 39, 1500-6.
  8. Tsai, W.W. et al. (2010) Nature 468, 927-32.
  9. Kikuchi, M. et al. (2009) Biochim Biophys Acta 1793, 1828-36.
  10. Allton, K. et al. (2009) Proc Natl Acad Sci U S A 106, 11612-6.
  11. Tai, E. and Benchimol, S. (2009) Proc Natl Acad Sci U S A 106, 11431-2.
  12. Jain, A.K. et al. (2014) Mol Cell Biol 34, 2695-709.
  13. Li, H. et al. (2012) PLoS One 7, e37657.
  14. Zhang, L.H. et al. (2015) Oncogene 34, 600-10.
  15. Fang, Z. et al. (2017) Oncol Lett 13, 1797-1806.
  16. Lv, D. et al. (2017) Nat Commun 8, 1454.
  17. Zhu, Y. et al. (2018) Exp Cell Res 367, 274-281.
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