Tie2 (AB33) Mouse mAb #4224
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Tie2 (AB33) Mouse mAb detects endogenous levels of Tie2 in various endothelial cell lines. It does not cross-react with related proteins.
Species Reactivity:
Human, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant Tie2 protein fragments corresponding to the extracellular domain of human Tie2.
Background
Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells. It is critical for vasculogenesis and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). A family of ligands known as the angiopoietins binds to Tie2. Interestingly, these ligands appear to have opposing actions; Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2; Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4,5). Downstream signaling components, including Grb2, Grb7, Grb14, SHP-2, the p85 subunit of phosphatidylinositol 3-kinase, and p56/Dok-2 interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains (6,7). Tyr992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).
- Ward, N.L. and Dumont, D.J. (2002) Semin. Cell Dev. Biol. 13, 19-27.
- Jones, N. and Dumont, D.J. (2000) Cancer Metastasis Rev. 19, 13-17.
- Partanen, J. and Dumont, D.J. (1999) Curr. Top. Microbiol. Immunol. 237, 159-172.
- Ellis, L. M. et al. (2002) Oncology 16, 31-35.
- Koh, G. Y. et al. (2002) Exp. Mol. Med. 34, 1-11.
- Jones, N. et al. (1999) J. Biol. Chem. 274, 30896-30905.
- Jones, N. et al. (2003) Mol. Cell. Biol. 23, 2658-2668.
- Murray, B. W. et al. (2001) Biochem. 40, 10243-10253.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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