R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TIE1 (D2K2T) Rabbit mAb #23111
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135, 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TIE1 (D2K2T) Rabbit mAb recognizes endogenous levels of total TIE1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TIE1 protein.
Background
TIE1 belongs to the Tie family of receptor tyrosine kinases. TIE1 is structurally similar to its homolog TIE2, but differs from the latter in that it does not have a known ligand and is thus considered an orphan receptor (1). A key function of TIE1 is to modulate TIE2 signaling, via heterodimerization with TIE2 at the cell surface (2). The effects of TIE1-TIE2 interaction are context-dependent; heterodimerization can either promote or inhibit downstream TIE2 signaling depending on localized TIE2 levels (3-6). Research studies have shown TIE1 to be implicated in angiogenesis, vascular maturation, tissue remodeling, and inflammation. Increased expression of TIE1 has also been associated with cancer stemness and atherosclerosis formation (7, 8).
- Merenmies, J. et al. (1997) Cell Growth Differ 8, 3-10.
- Seegar, T.C. et al. (2010) Mol Cell 37, 643-55.
- Korhonen, E.A. et al. (2016) J Clin Invest 126, 3495-510.
- Kim, M. et al. (2016) J Clin Invest 126, 3511-25.
- Yuan, H.T. et al. (2007) FASEB J 21, 3171-83.
- Savant, S. et al. (2015) Cell Rep 12, 1761-73.
- Torigata, M. et al. (2017) Cancer Med 6, 1378-1388.
- Woo, K.V. and Baldwin, H.S. (2011) Trends Cardiovasc Med 21, 118-23.
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