R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TIA-1 (D1Q3K) Rabbit mAb #86050
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42, 44 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TIA-1 (D1Q3K) Rabbit mAb recognizes endogenous levels of total TIA-1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIA-1 protein.
Background
T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, COX-2, cytochrome c, GADD45α, and HIF-1α (8-13).
- Tian, Q. et al. (1991) Cell 67, 629-39.
- Kawakami, A. et al. (1992) Proc Natl Acad Sci U S A 89, 8681-5.
- Izquierdo, J.M. and Valcárcel, J. (2007) J Biol Chem 282, 19410-7.
- Beck, A.R. et al. (1996) Nucleic Acids Res 24, 3829-35.
- Kedersha, N.L. et al. (1999) J Cell Biol 147, 1431-42.
- Gilks, N. et al. (2004) Mol Biol Cell 15, 5383-98.
- Eisinger-Mathason, T.S. et al. (2008) Mol Cell 31, 722-36.
- Gottschald, O.R. et al. (2010) J Mol Cell Biol 2, 345-56.
- Gueydan, C. et al. (1999) J Biol Chem 274, 2322-6.
- Piecyk, M. et al. (2000) EMBO J 19, 4154-63.
- Kawai, T. et al. (2006) Mol Cell Biol 26, 3295-307.
- Dixon, D.A. et al. (2003) J Exp Med 198, 475-81.
- Lal, A. et al. (2006) Mol Cell 22, 117-28.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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