Tensin 2 Antibody #11990
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 145-155 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Tensin 2 Antibody recognizes endogenous levels of total Tensin 2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg155 of human Tensin 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Tensin 2 belongs to the Tensin family of cytoskeletal proteins that includes Tensin 1-3 and Cten, which couple integrins to the actin cytoskeleton (1). Tensin proteins contain SH2 and phosphotyrosine binding (PTB) domains, which enable interaction with diverse signaling molecules and proteins. Tensin family proteins play important roles in signal transduction, cell proliferation, and motility (2-5).
Tensin 2 is localized to focal adhesions of various tissues with highest expression in the heart, kidney, and liver (6,7). Tensin 2 inhibits Akt/PKB signaling via a phosphatase tensin-type domain (8). However, Tensin 2 also mediates thrombopoietin/c-Mpl signaling, which promotes Akt signaling (9). Interaction with Tensin 2 is essential for the tumor suppressor function of Deleted in Cancer 1 (DLC1) (6,10,11).
Tensin 2 is localized to focal adhesions of various tissues with highest expression in the heart, kidney, and liver (6,7). Tensin 2 inhibits Akt/PKB signaling via a phosphatase tensin-type domain (8). However, Tensin 2 also mediates thrombopoietin/c-Mpl signaling, which promotes Akt signaling (9). Interaction with Tensin 2 is essential for the tumor suppressor function of Deleted in Cancer 1 (DLC1) (6,10,11).
- Lo, S.H. et al. (1994) Bioessays 16, 817-23.
- Lo, S.H. et al. (1994) J Cell Biol 125, 1067-75.
- Chen, H. and Lo, S.H. (2003) Biochem J 370, 1039-45.
- Katz, M. et al. (2007) Nat Cell Biol 9, 961-9.
- Chen, H. et al. (2002) Proc Natl Acad Sci U S A 99, 733-8.
- Clark, K. et al. (2010) J Cell Biochem 109, 808-17.
- Hafizi, S. et al. (2002) Biochem Biophys Res Commun 299, 793-800.
- Hafizi, S. et al. (2005) FASEB J 19, 971-3.
- Jung, A.S. et al. (2011) Cell Cycle 10, 1838-44.
- Chan, L.K. et al. (2009) PLoS One 4, e5572.
- Chen, L. et al. (2012) J Biol Chem 287, 26104-14.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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