R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TCEB2/Elongin B (F3G5G) Rabbit mAb #41892
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TCEB2/Elongin B (F3G5G) Rabbit mAb recognizes endogenous levels of total TCEB2/Elongin B protein. Based on sequence homology, this antibody is not predicted to cross-react with TCEB1/Elongin C protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp82 of human TCEB2/Elongin B protein.
Background
Transcription elongation factor B polypeptide 2 (TCEB2), also known as Elongin B, is a regulatory component of the SIII/Elongin elongation complex, which regulates elongation by RNA polymerase II by suppressing pausing of the polymerase during gene transcription. The SIII/Elongin complex is also composed of TCEB3/Elongin A, the catalytic subunit, and TCEB1/Elongin C, another regulatory subunit (1). TCEB2/Elongin B is part of the ubiquitin-proteasome pathway as a critical component of multiple E3 ligase complexes. It directly interacts with TCEB1/Elongin C as part of the VHL complex, which targets HIF-1α, a transcription factor that plays a critical role in the cellular response to hypoxia, for degradation (2,3). Dysregulation of HIF-α activity is associated with poor prognosis in multiple human cancers (4). TCEB2/Elongin B promotes therapy resistance in ovarian cancer (5,6), promotes breast cancer progression (7), and reduces radiosensitivity in triple-negative breast cancer (TNBC) (8). During HIV-1 infection, TCEB2/Elongin B is part of an E3 ligase complex that targets the anti-viral proteins APOBEC3F and APOBEC3G for degradation (9).
- Chen, Y. et al. (2023) Nat Struct Mol Biol 30, 1925-1935.
- Kibel, A. et al. (1995) Science 269, 1444-6.
- Kim, W.Y. and Kaelin, W.G. (2004) J Clin Oncol 22, 4991-5004.
- Jun, J.C. et al. (2017) Curr Sleep Med Rep 3, 1-10.
- Deng, Z. et al. (2024) Eur J Med Res 29, 456.
- Deng, Z. et al. (2016) Oncol Rep 35, 359-65.
- Sui, X.Y. et al. (2024) Cell Biol Toxicol 40, 24.
- Li, G. et al. (2024) Breast Cancer 31, 426-439.
- Bergeron, J.R. et al. (2010) PLoS Pathog 6, e1000925.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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