R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TACSTD2/TROP2 (E8Y8S) Rabbit mAb #47866
Filter:
- WB
- IP
- IF
- F
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 45-65 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Simple Western™ | 1:10 - 1:50 |
Immunoprecipitation | 1:50 |
Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Flow Cytometry (Live) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #72384.
For a carrier free (BSA and azide free) version of this product see product #72384.
Protocol
Specificity / Sensitivity
TACSTD2/TROP2 (E8Y8S) Rabbit mAb recognizes endogenous levels of total TACSTD2/TROP2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val90 of human TACSTD2/TROP2 protein.
Background
TROP2 is a transmembrane glycoprotein encoded by gene TACSTD2 (tumor-associated calcium signal transducer 2). TROP2 was first discovered as a biomarker of invasive trophoblast cells and later reported in many types of cancer cells, in various organs during development, and adult stem cells during homeostasis (1,2). TROP2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain, and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site and PKC phosphorylation site (Ser303) (1-4). TROP2 functions by regulating multiple signaling pathways, including the interaction of its extracellular domain with integrin beta1 to regulate FAK signaling, the association of its transmembrane domain with Claudin-1 and Claudin-7 for tight junction formation, and the regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway (1,2,5-8). All these functions are important for its role in tumor proliferation, metastasis, and invasion (1,2). PKC can phosphorylate TROP2 at Ser303; the phosphorylation changes the cytoplasmic tail conformation and further promotes its signaling (9). TROP2 can be activated through intramembrane proteolysis first by TACE, followed by further cleavage by Presenilin 1 and Presenilin 2. The proteolysis process is required for its role in tumor cell proliferation (10,11).
- McDougall, A.R. et al. (2015) Dev Dyn 244, 99-109.
- Shvartsur, A. and Bonavida, B. (2015) Genes Cancer 6, 84-105.
- El Sewedy, T. et al. (1998) Int J Cancer 75, 324-30.
- Linnenbach, A.J. et al. (1993) Mol Cell Biol 13, 1507-15.
- Trerotola, M. et al. (2013) Cancer Res 73, 3155-67.
- Trerotola, M. et al. (2015) Oncotarget 6, 14318-28.
- Nakatsukasa, M. et al. (2010) Am J Pathol 177, 1344-55.
- Cubas, R. et al. (2010) Mol Cancer 9, 253.
- Pavšič, M. et al. (2015) Sci Rep 5, 10324.
- Stoyanova, T. et al. (2012) Genes Dev 26, 2271-85.
- Ju, X. et al. (2016) Cancer Res 76, 6723-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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