T7-Tag (F1O6G) Mouse mAb #83159
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Recombinant and Transfected |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
T7-Tag (F1O6G) Mouse mAb detects recombinant proteins containing the T7 epitope tag. The antibody recognizes the T7-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. T7-Tag (F1O6G) Mouse mAb detects exogenously expressed T7-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect several non-specific bands of unknown origin in rat cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The T7-tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7-tag has been used extensively as a general epitope tag in many expression vectors, including the pET system that is based on T7 RNA polymerase expression systems (1).
The T7-tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7-tag has been used extensively as a general epitope tag in many expression vectors, including the pET system that is based on T7 RNA polymerase expression systems (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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