T-bet/TBX21 (V365) Antibody #5214
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
T-bet/TBX21 (V365) Antibody detects endogenous levels of total T-bet protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val365 of human T-bet/TBX21 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11).
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11).
- Wilkinson, D.G. et al. (1990) Nature 343, 657-9.
- Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19.
- Showell, C. et al. (2004) Dev Dyn 229, 201-18.
- Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70.
- Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60.
- Baldini, A. (2004) Curr Opin Cardiol 19, 201-4.
- Abrahams, A. et al. (2010) IUBMB Life 62, 92-102.
- Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9, 109-18.
- Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.
- Ho, I.C. and Glimcher, L.H. (2002) Cell 109 Suppl, S109-20.
- Peng, S.L. (2006) Cell Mol Immunol 3, 87-95.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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