R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
T-bet/TBX21 (D6N8B) XP® Rabbit mAb #13232
Filter:
- WB
- IP
- IHC
- IF
- F
- ChIP
- C&R
- C&T
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58-68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #27112.
For a carrier free (BSA and azide free) version of this product see product #27112.
Protocol
Specificity / Sensitivity
T-bet/TBX21 (D6N8B) XP® Rabbit mAb recognizes endogenous levels of total T-bet/TBX21 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein.
Background
The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11).
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11).
- Wilkinson, D.G. et al. (1990) Nature 343, 657-9.
- Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19.
- Showell, C. et al. (2004) Dev Dyn 229, 201-18.
- Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70.
- Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60.
- Baldini, A. (2004) Curr Opin Cardiol 19, 201-4.
- Abrahams, A. et al. (2010) IUBMB Life 62, 92-102.
- Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9, 109-18.
- Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.
- Ho, I.C. and Glimcher, L.H. (2002) Cell 109 Suppl, S109-20.
- Peng, S.L. (2006) Cell Mol Immunol 3, 87-95.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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