R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SYVN1 (D3O2A) Rabbit mAb #14773
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SYVN1 (D3O2A) Rabbit mAb recognizes endogenous levels of total SYVN1 protein. This antibody does not cross-react with AMFR protein, but may cross-react with an unidentified protein of 60 kDa.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SYVN1 protein.
Background
Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6).
- Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
- Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
- Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
- Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
- Amano, T. et al. (2003) Genes Dev 17, 2436-49.
- Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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