R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Syntenin-1/MDA9 (E2I9L) Rabbit mAb (BSA and Azide Free) #32485
Filter:
- WB
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #27964. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #27964
For standard formulation of this product see product #27964
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Syntenin-1/MDA9 (E2I9L) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total syntenin-1/MDA9 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human syntenin-1/MDA9 protein.
Background
Syntenin-1 (MDA9, SDCBP) is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGFβR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers, and has been linked to growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway which has been shown to directly contribute to the formation and internalization of exosomes (9-11).
- Pradhan, A.K. et al. (2020) Cancer Metastasis Rev 39, 769-781.
- Dasgupta, S. et al. (2013) Clin Cancer Res 19, 4621-33.
- Das, S.K. et al. (2012) Front Biosci (Landmark Ed) 17, 1-15.
- Menezes, M.E. et al. (2016) Oncotarget 7, 80175-80189.
- Das, S.K. et al. (2018) Cancer Res 78, 2852-2863.
- Das, S.K. et al. (2019) Adv Cancer Res 144, 137-191.
- Kegelman, T.P. et al. (2015) Expert Opin Ther Targets 19, 97-112.
- Shimada, T. et al. (2019) Int J Mol Sci 20, 4171. doi: 10.3390/ijms20174171.
- Fares, J. et al. (2017) Cell Adh Migr 11, 124-126.
- Baietti, M.F. et al. (2012) Nat Cell Biol 14, 677-85.
- Kugeratski, F.G. et al. (2021) Nat Cell Biol 23, 631-641.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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