Syntaxin 6 (C34B2) Rabbit mAb #2869
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 32 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr140 of mouse syntaxin 6 protein.
Background
Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10).
- Bock, J.B. et al. (2001) Nature 409, 839-841.
- Bock, J.B. et al. (1996) J. Biol. Chem. 271, 17961-17965.
- Wendler, F. and Tooze, S. (2001) Traffic 2, 606-611.
- Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261-1271.
- Mallard, F. et al. (2002) J. Cell Biol. 156, 653-664.
- Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946-2958.
- Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973-986.
- Martín-Martín, B. et al. (2000) Blood 96, 2574-25783.
- Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699-1709.
- Kuliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690-1701.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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