Render Target: SSR
Render Timestamp: 2024-12-24T12:39:19.182Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:19:58.496
Product last modified at: 2024-12-13T00:00:08.406Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. BAF Complex Antibody Sampler Kit

BAF Complex Antibody Sampler Kit #12854

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The BAF Complex Antibody Sampler Kit provides an economical means of detecting total protein from the SWI/SNF family members including ARID1A/BAF250A, Brg1, BRM, SMARCC1/BAF155, SMARCC2/BAF170 and SMARCB1/BAF47. The kit contains enough primary antibody to perform two western blots per primary antibody.

    Specificity / Sensitivity

    Each antibody in this kit recognizes endogenous levels of total protein for the specified target and does not cross-react with other family members. ARID1A/BAF250A (D2A8U) Rabbit mAb also cross-reacts with proteins of unknown origin at 65 kDa.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1293 of human ARID1A/BAF250A protein, Gly264 of human BRM protein, Gly975 of human SMARCC1/BAF155 protein, Ile818 of human SMARCC2/BAF170 protein, or Leu120 of human SMARCB1/BAF47 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala52 of human Brg1 protein. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

    Background

    ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13). SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/BAF47 are members of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro (14). ARID1A/BAF250A is one of the accessory subunits of the SWI/SNF complex (15). SMARCC1, SMARCB1, and ARID1A are an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF). SMARCC1 is necessary for early embryogenesis, especially proper brain and visceral endoderm development (16-18). SMARCB1 is necessary for early embryogenesis and hepatocyte differentiation (19,20). ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes (15). While SMARCC2 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2 leads to loss of stem cell pluripotency and self renewal (21).
    1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
    2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
    3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
    4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
    5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
    6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
    7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
    8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
    9. Simone, C. (2006) J Cell Physiol 207, 309-14.
    10. Yamamichi, N. et al. (2005) Oncogene 24, 5471-81.
    11. Reisman, D.N. et al. (2002) Oncogene 21, 1196-207.
    12. Shen, H. et al. (2008) Cancer Res 68, 10154-62.
    13. Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30.
    14. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
    15. Gao, X. et al. (2008) Proc Natl Acad Sci U S A 105, 6656-61.
    16. Han, D. et al. (2008) Dev Biol 315, 136-46.
    17. Kim, J.K. et al. (2001) Mol Cell Biol 21, 7787-95.
    18. Schaniel, C. et al. (2009) Stem Cells 27, 2979-91.
    19. Klochendler-Yeivin, A. et al. (2000) EMBO Rep 1, 500-6.
    20. Gresh, L. et al. (2005) EMBO J 24, 3313-24.
    21. Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6.

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