R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
STK25 (E7T2F) Rabbit mAb #31856
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
STK25 (E7T2F) Rabbit mAb recognizes endogenous levels of total STK25 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human STK25 protein.
Background
STK25 (Serine/threonine protein kinase 25, YSK, SOK1) belongs to the germinal center kinase III (GCKII) subfamily of sterile-20 (STE20) protein kinases (1). STK25 is highly conserved across metazoans, and is reported to be involved in various core cellular processes, including cell migration (2,3) and metabolism (4,5). Along with the closely related MST3 and MST4 kinases, STK25 has been identified as a component of the striatin-interacting phosphatase and kinase (STRIPAK) complex (6). Its role in this regard is one of the mechanisms by which it has been reported to regulate the Hippo signaling pathway (7,8). A defined role for STK25 in lipid metabolism (9) has led some to consider targeting STK25 for the treatment of lipid-related metabolic diseases (10), including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) (11,12).
- Osada, S. et al. (1997) Oncogene 14, 2047-57.
- Matsuki, T. et al. (2021) J Neurosci 41, 8887-8903.
- Preisinger, C. et al. (2004) J Cell Biol 164, 1009-20.
- Kurhe, Y. et al. (2021) Cell Mol Gastroenterol Hepatol 13, 405-423.
- Zhang, Y. et al. (2022) Cancer Cell Int 22, 4.
- Kean, M.J. et al. (2011) J Biol Chem 286, 25065-75.
- Bae, S.J. et al. (2020) Elife 9, e54863. doi: 10.7554/eLife.54863.
- Lim, S. et al. (2019) Nat Commun 10, 1547.
- Nerstedt, A. et al. (2020) J Lipid Res 61, 178-191.
- Cansby, E. et al. (2020) JCI Insight 5, e140483. doi: 10.1172/jci.insight.140483.
- Kühnel, F. (2021) Cell Mol Gastroenterol Hepatol 13, 676-677.
- Cansby, E. et al. (2019) Cell Mol Gastroenterol Hepatol 7, 597-618.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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