Render Target: SSR
Render Timestamp: 2024-11-28T11:05:22.318Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-04-05 20:29:16.041
Product last modified at: 2024-05-30T07:07:14.004Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

STING Antibody #3337

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Inquiry Info. # 3337

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    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 35
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    STING Antibody recognizes endogenous levels of total STING protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu325 of human STING protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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