R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SRCAP (E9V8O) Rabbit mAb #28716
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 400 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SRCAP (E9V8O) Rabbit mAb recognizes endogenous levels of total SRCAP protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro911 of human SRCAP protein.
Background
Snf2-Related CBP Activator Protein (SRCAP) is an ATPase that exists in a large complex that activates transcription by enhancing CBP activity and by depositing H2A.Z into nucleosomes (1-3). SRCAP can enhance nuclear receptor function along with CARM-1 and GRIP-1 (4). In prostate cancer, SRCAP associates with androgen receptor to control prostate specific antigen expression and increase cell growth (5). During double-stranded break repair, SRCAP is required for DNA-end resection and RAD51 recruitment (6). Mutations in SRCAP result in a developmental disorder called Floating-Harbor syndrome (7,8). SRCAP also plays a role in viral infection as it has been shown to interact with various viral proteins (9,10).
- Johnston, H. et al. (1999) J Biol Chem 274, 16370-6.
- Monroy, M.A. et al. (2001) J Biol Chem 276, 40721-6.
- Ruhl, D.D. et al. (2006) Biochemistry 45, 5671-7.
- Monroy, M.A. et al. (2003) Mol Endocrinol 17, 2519-28.
- Slupianek, A. et al. (2010) J Cell Physiol 224, 369-75.
- Dong, S. et al. (2014) Curr Biol 24, 2097-2110.
- Hood, R.L. et al. (2012) Am J Hum Genet 90, 308-13.
- Le Goff, C. et al. (2013) Hum Mutat 34, 88-92.
- Ghosh, A.K. et al. (2000) J Biol Chem 275, 7184-8.
- Xu, X. et al. (2001) J Virol 75, 10033-40.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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