渲染靶标:SSR
Render Timestamp: 2025-01-09T03:26:32.704Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:53:36.513
Product last modified at: 2025-01-01T09:05:34.102Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Spry1 (D9V6I) Rabbit mAb #12993

Filter:
  • WB
  • IP
Western Blotting Image 1: Spry1 (D9V6I) Rabbit mAb
Western blot analysis of extracts from various cell lines using Spry1 (D9V6I) Rabbit mAb.

To Purchase # 12993T

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 35
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Spry1 (D9V6I) Rabbit mAb recognizes endogenous levels of total Spry1 protein. This antibody may also cross-react with an unidentified protein at 65 kDa.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val145 of human Spry1 protein.

Background

Spry1 is a member of the Sprouty (Spry) family proteins that was initially identified in Drosophila as an inhibitor of the FGF signaling pathway (1). There are four human Spry proteins (Spry1-4), encoded by different genes, and they all share a highly conserved carboxy-terminal cystine-rich Spry domain that is known to be essential for their receptor tyrosine kinase inhibitory function stimulated by various growth factors (1-3). Spry1 and other Spry proteins play a key role in embryonic development, tissue and organ formation, as well as growth in almost all living organisms (1-4). Spry proteins are considered tumor suppressors due to their inhibitory function in a variety of growth factor signaling pathways (2,3). Spry1 anchors itself to the membrane by palmitoylation and can translocate from the cytosol to the membrane by binding to caveolin-1 (5,6). Regulation of Spry1 protein function is thought to occur at various levels. Spry1 regulation includes transcriptional regulation by growth factors and kinases (1,4,7), post-transcriptional regulation by microRNA-21 (8), post-translational modifications including phosphorylation, dephosphorylation, ubiquitination and proteasomal degradation, and regulation by its interacting protein partners (2,3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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