R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Spred1 (D6D8B) Rabbit mAb #94063
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Spred1 (D6D8B) Rabbit mAb recognizes endogenous levels of total Spred1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln281 of human Spred1 protein.
Background
Spred1 belongs to the Spred family functioning as sprouty-related suppressor of Ras signaling down stream of growth receptor signaling (1,2). The protein has a N-terminal EVH1 (Ena/VASP homology-1) domain, followed by a KBD (c-Kit- binding domain) and a C-terminal SPR (Sprouty) domain. The C-terminal SPR domain functions to translocate Spred1 to the membrane following growth factor stimulation (1,3). The N-terminal EVH1 domain interacts with neurofibromin to bring the later to the membrane proximal signaling complex for down regulation of Ras signaling (4,5). Mutations of Spred1 are the causes of Legius Syndrome (6,7). Spred1 expression inhibits tumor cell motility and metastasis, and down regulation has been found in hepatocarcinoma and myeloblastic leukemia (8-10).
- Wakioka, T. et al. (2001) Nature 412, 647-51.
- Kato, R. et al. (2003) Biochem Biophys Res Commun 302, 767-72.
- King, J.A. et al. (2005) Biochem J 388, 445-54.
- Stowe, I.B. et al. (2012) Genes Dev 26, 1421-6.
- Hirata, Y. et al. (2015) J Biol Chem , .
- Brems, H. et al. (2007) Nat Genet 39, 1120-6.
- Brems, H. and Legius, E. (2013) Keio J Med 62, 107-12.
- Miyoshi, K. et al. (2004) Oncogene 23, 5567-76.
- Yoshida, T. et al. (2006) Oncogene 25, 6056-66.
- Pasmant, E. et al. (2015) Oncogene 34, 631-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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