R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Spi-C (E7V7F) Rabbit mAb #41910
Filter:
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 25, 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Spi-C (E7V7F) Rabbit mAb recognizes overexpressed levels of total Spi-C protein. This antibody detects a 70 kDa band of unknown origin in some cell lines.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala205 of mouse Spi-C protein.
Background
Spi-C belongs to the erythroblast transformation-specific (ETS) domain family of transcription factors and binds to the purine-rich PU-box (1-3). Spi-C is a lineage-instructive factor involved in generating mature, specialized subsets of both myeloid and lymphoid cells (6). Spi-C is highly expressed by red pulp macrophages in the spleen, which are required for red blood cell recycling and iron homeostasis (4,6). Spi-C is also transiently expressed in B cells during specific stages of their development and regulates cell fate decisions at the pro-B to pre-B cell stage and plasma cell differentiation (1-3,6). Spi-C has also been shown to be a positive regulator that promotes osteoclast differentiation and function (5).
- Bemark, M. et al. (1999) J Biol Chem 274, 10259-67.
- Hashimoto, S. et al. (1999) Int Immunol 11, 1423-9.
- Carlsson, R. et al. (2003) Mol Immunol 39, 1035-43.
- Kohyama, M. et al. (2009) Nature 457, 318-21.
- Go, E.M. et al. (2020) Exp Mol Med 52, 691-701.
- Raczkowski, H.L. and DeKoter, R.P. (2021) WIREs Mech Dis 13, e1519.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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