R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SPHK1 (D1H1L) Rabbit mAb #12071
Filter:
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 45-60 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SPHK1 (D1H1L) Rabbit mAb recognizes endogenous levels of total SPHK1 protein. This antibody also cross-reacts with a protein of unknown origin at 160 kDa in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro374 of human SPHK1 protein.
Background
Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.
- Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
- Xia, P. et al. (2000) Curr Biol 10, 1527-30.
- Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
- Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
- Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
- Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
- Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
- Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
- Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
- Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
- Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
- Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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