R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit mAb #63335
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Sphingosine-1-Phosphate Receptor 1 (E8U3O) Rabbit mAb recognizes endogenous levels of total sphingosine-1-phosphate receptor 1 protein. Nonspecific nuclear background may be observed by immunofluorescence in some cell lines, especially with higher than recommended concentrations of antibody.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly366 of human sphingosine-1-phosphate receptor 1 protein.
Background
Sphingosine-1-phosphate receptor 1 (S1PR1, EDG1) is a G protein-coupled receptor (GPCR), one of five members (S1PR1-5) belonging to the sphingosine-1-phosphate receptor (S1PR) family (1,2). This family of GPCRs has been shown to exhibit broad and often overlapping expression patterns across a wide array of tissues. The cognate ligand for S1PRs is sphingosine-1-phosphate (S1P), a phosphorylated lipid signaling molecule derived from sphingosine. S1P/S1PR binding can trigger the activation and/or inhibition of various downstream signaling pathways, depending on context. Pathways identified downstream of S1P/S1PR include PI3K/Akt, PLC, Ras/MAPK and PKA (3,4). S1P/S1PR1 signaling pathways play important roles in many basic cellular functions during normal development, including chemotaxis, vascular integrity, and immune cell egress, but can also contribute to a variety of pathological conditions, including atherogenesis, multiple sclerosis, and tumorigenesis (5-9).
- Pérez-Jeldres, T. et al. (2021) Drugs 81, 985-1002.
- Chun, J. et al. (2021) Drugs 81, 207-231.
- Pan, Y. et al. (2021) Clin Exp Pharmacol Physiol 48, 637-650.
- Xiao, L. et al. (2019) Front Immunol 10, 1409.
- Rivera, J. et al. (2008) Nat Rev Immunol 8, 753-63.
- Anwar, M. and Mehta, D. (2020) Biochim Biophys Acta Mol Cell Biol Lipids 1865, 158760.
- Song, J.H. et al. (2021) Biomol Ther (Seoul) 29, 373-383.
- Roy, R. et al. (2021) CNS Drugs 35, 385-402.
- Anu, B. et al. (2021) Adv Protein Chem Struct Biol 125, 259-274.
限制使用
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