R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SPDEF (F9M9S) Rabbit mAb #29721
Filter:
- WB
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37-45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SPDEF (F9M9S) Rabbit mAb recognizes endogenous levels of total SPDEF protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human SPDEF protein.
Background
SAM pointed domain-containing Ets transcription factor (SPDEF), also known as prostate-derived Ets factor (PDEF), was originally identified as an androgen-independent regulator of prostate-specific antigen (PSA) and binds to 5'-GGAT-3' DNA sequences (1). Expressed primarily in epithelial cells, SPDEF acts as a transcriptional regulator of epithelial cell adhesion, structure, and differentiation. SPDEF is tightly regulated across a variety of cancer types and has been reported as both a tumor oncogene and tumor supressor (2,3). In luminal breast cancer, SPDEF is overexpressed and promotes GALNT7 transcription, increasing tumor cell proliferation and stemness (4). SPDEF suppresses colorectal cancer by inhibiting β-catenin and downstream expression of cyclin D1 and c-Myc (5). Methylation of SPDEF gene enhancer regions by DNA methyltransferases (DNMTs) suppresses SPDEF expression, driving the observed differences in SPDEF transcripts and protein among prostate cancer cell lines and tumor grades (6).
- Oettgen, P. et al. (2000) J Biol Chem 275, 1216-25.
- Bao, K.C. and Wang, F.F. (2022) Neoplasma 69, 1270-1276.
- Ye, T. et al. (2020) Cancer Manag Res 12, 3891-3902.
- Li, J. et al. (2023) Cell Death Dis 14, 569.
- Noah, T.K. et al. (2013) Gastroenterology 144, 1012-1023.e6.
- Vatanmakanian, M. et al. (2023) Front Endocrinol (Lausanne) 14, 1156120.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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