R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SOX5 (E8S4A) Rabbit mAb #25792
Filter:
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Chromatin IP | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SOX5 (E8S4A) Rabbit mAb detects endogenous levels of SOX5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SOX5 protein.
Background
SOX5 is a member of the SOX (SRY-related HMG-box) family of transcription factors. It is involved in the regulation of embryonic development, and specifically essential for driving chondrogenesis (1). In recent years, it has become apparent that SOX5 plays a significant role in the progression of various tumor types, such as breast cancer, glioma, hepatocellular carcinoma, lung cancer, and others (2-5). Upregulation of SOX5 in these tumor types has been reported to drive metastasis through activation of epithelial-mesenchymal transition (EMT) (2-5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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