R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Sox17 (D1T8M) Rabbit mAb #81778
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Sox17 (D1T8M) Rabbit mAb recognizes endogenous levels of total Sox17 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro272 of human Sox17 protein.
Background
SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins do not directly mediate transcription, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1).
Transcription factor Sox17 is a Sox family protein with an established role in endoderm specification during development (2). In addition, Sox17 plays essential roles in the maintenance of the hematopoietic stem cell pool (3) and for vascular morphogenesis during development (4,5). Sox17 negatively regulates oligodendrocyte precursor differentiation by antagonizing β-catenin signaling (6). Mutation in the corresponding Sox17 gene in humans is associated with a form of vesicoureteral reflux, a disorder characterized by congenital kidney and urinary tract defects (7).
Transcription factor Sox17 is a Sox family protein with an established role in endoderm specification during development (2). In addition, Sox17 plays essential roles in the maintenance of the hematopoietic stem cell pool (3) and for vascular morphogenesis during development (4,5). Sox17 negatively regulates oligodendrocyte precursor differentiation by antagonizing β-catenin signaling (6). Mutation in the corresponding Sox17 gene in humans is associated with a form of vesicoureteral reflux, a disorder characterized by congenital kidney and urinary tract defects (7).
- Kamachi, Y. and Kondoh, H. (2013) Development 140, 4129-44.
- Engert, S. et al. (2013) Development 140, 3128-38.
- Clarke, R.L. et al. (2013) Nat Cell Biol 15, 502-10.
- Lange, A.W. et al. (2014) Dev Biol 387, 109-20.
- Choi, E. et al. (2012) Stem Cells 30, 2297-308.
- Chew, L.J. et al. (2011) J Neurosci 31, 13921-35.
- Gimelli, S. et al. (2010) Hum Mutat 31, 1352-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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