R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SNX5 (E4Q2X) Rabbit mAb #39138
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SNX5 (E4Q2X) Rabbit mAb recognizes endogenous levels of total SNX5 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu216 of human SNX5 protein.
Background
Sorting nexins (SNXs) are a family of cytoplasmic proteins characterized by the presence of a phosphatidylinositol 3-phosphate (PI3P) binding phox (PX) domain. This binding occurs mainly in the early endosome and allows for trafficking of the bound protein to either a degradative or recycling pathway (1). The sorting nexin family member SNX5 was identified in binding studies with Fanconi anemia complementation group A (FANCA) protein (2). In addition to the PX domain, STX5 has a Bin/Amphiphysin/Rvs (BAR) domain that can sense membrane curvature and induce membrane tubulation (3). Aberrant expression of SNX5 can contribute to tumorigenesis, invasion, and metastasis of several human cancers (4-7). Elevated expression of SNX5 has been reported in Parkinson’s disease, where it may have a role in promoting ferroptosis (8). Studies have found that SNX5 has an essential role in macropinocytosis (9). In addition, SNX5 is part of a complex involved in recognizing membrane curvature on autolysosomes and regulating recycling of autophagosomal components (10). In a genome-wide siRNA, SNX5 was found to be an essential protein involved in virus-mediated autophagy and immunity (11).
- Hanley, S.E. and Cooper, K.F. (2020) Cells 10,17. doi: 10.3390/cells10010017.
- Otsuki, T. et al. (1999) Biochem Biophys Res Commun 265, 630-5.
- van Weering, J.R. et al. (2010) Semin Cell Dev Biol 21, 371-80.
- Zhou, Q. et al. (2020) Oncogene 39, 2140-2155.
- Jitsukawa, S. et al. (2017) J Pathol 243, 342-353.
- Ara, S. et al. (2012) Cancer Sci 103, 1356-62.
- Zhou, Q. et al. (2022) Mol Ther Oncolytics 24, 87-100.
- Huang, Z. et al. (2022) Oxid Med Cell Longev 2022, 5463134.
- Lim, J.P. et al. (2008) BMC Cell Biol 9, 58.
- Zhou, C. et al. (2022) Nat Cell Biol 24, 497-512.
- Dong, X. et al. (2021) Nature 589, 456-461.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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