R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SNAT1/SLC38A1 (D9L2P) Rabbit mAb #36057
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50-70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SNAT1/SLC38A1 (D9L2P) Rabbit mAb recognizes endogenous levels of total SNAT1/SLC38A1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly37 of human SNAT1/SLC38A1 protein.
Background
SNAT1/SLC38A1 belongs to the system A transporters that mediate Na+-dependent transport of short-chain neutral amino acids such as alanine, serine, and glutamine. SNAT1/SLC38A1 mediates the uptake of glutamine in neurons and plays a crucial role in glutamate-glutamine cycle. Steep concentration gradients across the plasma membrane are achieved by coupling of the electrochemical sodium gradient to amino acid transport. This allows a unidirectional mode of transport for SNAT1/SLC38A1. Upregulation of SNAT1/SLC38A1 by neurotrophic factors is key to dendritic growth and branching of cortical neurons. High expression of SNAT1/SLC38A1 is found in cerebral cortex primarily in neurons and to a lesser extent in astrocytes (1-4). Elevated SNAT1/SLC38A1 expression is prominent in human solid tumors including gliomas, hepatocellular carcinomas and human breast cancer (5-8). Research studies show that an aberrant SNAT1/SLC38A1 expression profile correlates with solid tumor recurrence and poor prognosis in patients with cholangiocarcinoma (9).
- Yao, D. et al. (2000) J Biol Chem 275, 22790-7.
- Mackenzie, B. et al. (2003) J Biol Chem 278, 23720-30.
- Chaudhry, F.A. et al. (2002) J Cell Biol 157, 349-55.
- Yu, W.L. et al. (2011) J Surg Res 171, 663-8.
- Melone, M. et al. (2004) Cereb Cortex 14, 562-74.
- Kondoh, N. et al. (2007) Int J Oncol 31, 81-7.
- Sidoryk, M. et al. (2004) Neuroreport 15, 575-8.
- Wang, K. et al. (2013) BMC Cancer 13, 343.
- Burkhalter, J. et al. (2007) J Biol Chem 282, 5152-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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