R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SLP-2 (D2I9X) XP® Rabbit mAb #89199
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SLP-2 (D2I9X) XP® Rabbit mAb recognizes endogenous levels of total SLP-2 protein. Bands of unknown identity are detected by western blot at 80 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val182 of human SLP-2 protein.
Background
Stomatin-like protein 2 (SLP-2 and also known as STOML2) is a lipid-anchored mitochondrial protein that is part of a large protein complex that regulates mitochondrial biogenesis and function. Proteomic studies identified SLP-2 as a widely expressed mitochondria-enriched protein (1). As a member of both the stomatin family and stomatin-prohibitin-flotillin-HfLC/K (SPFH) superfamily of proteins, SLP-2 forms large hetero-oligomeric complexes with other mitochondrial proteins, including prohibtin, mitofusin 2, and cardiolipin (2, 3). SLP-2 contains a highly conserved SPFH domain that mediates its ability to associate with the mitochondrial inner membrane and form specialized membrane microdomains. As an inner membrane organizer of other mitochondrial proteins, SLP-2 performs multiple mitochondrial functions, including regulation of mitochondrial biogenesis, energy/calcium homeostasis, translation, and mitochondrial-mediated cellular stress responses (3, 4, 5, 6, 7, 8). Enhanced SLP-2 expression is also associated with several human cancers, including gallbladder, rectal, and gastric cancer (9, 10, 11).
- Da Cruz, S. et al. (2003) J Biol Chem 278, 41566-71.
- Hájek, P. et al. (2007) J Biol Chem 282, 5670-81.
- Christie, D.A. et al. (2011) Mol Cell Biol 31, 3845-56.
- Da Cruz, S. et al. (2010) Cell Calcium 47, 11-8.
- Wang, Y. et al. (2009) Cancer Biol Ther 8, 1651-8.
- Christie, D.A. et al. (2012) J Immunol 189, 4349-60.
- Tondera, D. et al. (2009) EMBO J 28, 1589-600.
- Mitsopoulos, P. et al. (2017) PLoS One 12, e0179967.
- Wang, W.X. et al. (2014) Tumour Biol 35, 419-23.
- Liu, Z. et al. (2014) Oncol Rep 31, 153-60.
- Bartolome, A. et al. (2016) APMIS 124, 271-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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