Sleeping Beauty Transposase Antibody #98923
Filter:
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Sleeping Beauty Transposase Antibody recognizes transfected levels of total Sleeping Beauty transposase protein.
Species Reactivity:
All Species Expected
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu258 of Sleeping Beauty transposase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Sleeping Beauty Transposase is part of a transposon system designed to allow viral free genetic insertion into vertebrate DNA. The system is composed of two components: a transposable element (transposon) that can carry DNA of interest, and a transposase that cuts and pastes the transposon into the genome. The transposase was identified from a consensus sequence of inactive Tc1/mariner-like transposase DNA sequences from salmonid fish. It was constructed by fusing and modifying two sequences from Atlantic salmon (Salmo salar) and one sequence from rainbow trout (Oncorhynchus mykiss). The transposon,T, was identified from a consensus sequence of extinct Tc-1 like transposons in salmonid fish (Tanichthys albonubes) (1). Further modifications of the system have been made since its initial construction: sequence changes to the transposase to fit better to an improved consensus sequence has increased its efficiency by 100 fold (2).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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