R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SLC25A1 (E7B2B) Rabbit mAb #97835
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:300 - 1:1200 |
| Immunofluorescence (Frozen) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SLC25A1 (E7B2B) Rabbit mAb recognizes endogenous levels of total SLC25A1 protein. Species cross-reactivity for IHC-P is human only.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the mitochondrial matrix-facing domains of human SLC25A1 protein. The antigen has been further characterized as corresponding to residues surrounding Arg173 of human SLC25A1 protein.
Background
Members of the solute carrier 25 (SLC25) family transport molecules over the mitochondrial inner membrane (1,2). Mutations in these carriers often result in rare but severe pathologies (3). SLC25A1 (tricarboxylate transport protein, CIC, or citrate transport protein) transports citrate from the inner mitochondrial matrix to the cytosol in exchange for malate. This function makes it a key source of cytoplasmic acetyl-CoA and downstream lipid biosynthesis (4). Mutations in SLC25A1 result in D-2- and L-2-hydroxyglutaric aciduria, causing lowered levels of citrate and isocitrate in urine, with elevated urinary levels of other TCA-cycle intermediates (3). Cancer cells have elevated mitochondrial membrane potential and require SLC25A1 to maintain and promote tumorigenesis (5,6). SLC25A1 is a transcriptional target of p53, and inhibiting SLC25A1 activity can blunt mutant p53-driven tumor growth (6). Inhibitors of SLC25A1 have been found to slow the growth of pancreatic and non-small cell lung cancers (7,8).
- Ruprecht, J.J. and Kunji, E.R.S. (2021) Annu Rev Biochem 90, 535-558.
- Kunji, E.R.S. et al. (2020) Physiology (Bethesda) 35, 302-327.
- Palmieri, F. et al. (2020) Biomolecules 10, 655. doi: 10.3390/biom10040655.
- Coleman, P.S. and Parlo, R.A. (2021) Front Cell Dev Biol 9, 626316.
- Rochette, L. et al. (2020) Molecules 25, 2417. doi: 10.3390/molecules25102417.
- Kolukula, V.K. et al. (2014) Oncotarget 5, 1212-25.
- Liu, J. et al. (2020) J Proteome Res 19, 3825-3836.
- Fernandez, H.R. et al. (2018) Cell Death Differ 25, 1239-1258.
限制使用
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专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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