R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SIGMAR2/TMEM97 (E8R4R) Rabbit mAb #62790
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 19 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SIGMAR2/TMEM97 (E8R4R) Rabbit mAb recognizes endogenous levels of total SIGMAR2/TMEM97 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro57 of human SIGMAR2/TMEM97 protein.
Background
Sigma intracellular receptor 2 (SIGMAR2 or TMEM97) is an endoplasmic reticulum (ER)-resident membrane chaperone protein that regulates the sterol transporter NPC1 (1). Recently, SIGMAR2/TMEM97 was identified as the opioid 2 receptor. Opioid receptors are a large super family of 7 transmembrane-spanning G protein-coupled receptors (GPCRs) that are activated by both endogenous opioid peptides and by exogenously administered opiate compounds (2). Opioid receptors are of pharmacological interest not only as analgesics but to treat neurological disorders including psychiatric and neurodegenerative diseases. Opioid receptors are classified pharmacologically, including σ1 and σ2 type receptors. Although SIGMAR1 was identified in 1996 as the σ1 receptor, identification of the σ2 type receptor remained elusive until 2017 when TMEM97 was identified as the σ2 type receptor (3,4). In addition to acting as an opioid receptor, SIGMAR2/TMEM97 may also act within a complex to regulate cellular update of Aβ, the pathological hallmark of Alzheimer's disease (5,6).
- Bartz, F. et al. (2009) Cell Metab 10, 63-75.
- Waldhoer, M. et al. (2004) Annu Rev Biochem 73, 953-90.
- Hanner, M. et al. (1996) Proc Natl Acad Sci U S A 93, 8072-7.
- Alon, A. et al. (2017) Proc Natl Acad Sci U S A 114, 7160-7165.
- Yang, K. et al. (2020) Molecules 25, 5439. doi: 10.3390/molecules25225439.
- Riad, A. et al. (2020) Mol Neurobiol 57, 3803-3813.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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