R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SIGMAR1 (D4J2E) Rabbit mAb #61994
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SIGMAR1 (D4J2E) Rabbit mAb recognizes endogenous levels of total SIGMAR1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg137 of human SIGMAR1 protein.
Background
Sigma non-opioid intracellular receptor 1 (SIGMAR1) is an endoplasmic reticulum (ER) membrane chaperone that forms raft-like microdomains on the ER, where it interacts with mitochondria at the mitochondria-associated ER membrane domain (MAM). At MAM, SIGMAR1 maintains proper ER-mitochondrion Ca2+ signaling, regulates mitochondria function, and enhances cellular survival upon ER stress (1-4). When activated, SIGMAR1 translocates to ER and plasma membrane, where it interacts with a plethora of membrane proteins, including ion channels, neurotransmitter receptors, and kinases. SIGMAR1 also modulates a variety of neuronal functions, such as neuronal excitability, neuroplasticity, neuroprotection, and neurorestoration (5-7). SIGMAR1 binds to many anti-psychotic drugs and it is implicated in addiction, pain, neurodegenerative diseases, and depression (8-11). Recently, mutations in the SIGMAR1 gene have been reported to be associated with amyotrophic lateral sclerosis (12,13). Besides its important roles in central nervous system and peripheral nervous system, SIGMAR1 also enhances cancer cell migration and invasion (14,15).
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- Liu, L.L. et al. (2016) Neuroscience 332, 53-60.
- Miyatake, R. et al. (2004) Biol Psychiatry 55, 85-90.
- Choi, S.R. et al. (2017) J Pain 18, 415-427.
- Huang, Y. et al. (2011) Curr Alzheimer Res 8, 765-70.
- Mandelli, L. et al. (2017) Adv Ther 34, 713-724.
- Fukunaga, K. et al. (2015) J Pharmacol Sci 127, 36-41.
- Al-Saif, A. et al. (2011) Ann Neurol 70, 913-9.
- Gueguinou, M. et al. (2017) Oncogene 36, 3640-7.
- Crottès, D. et al. (2016) Cancer Res 76, 607-18.
限制使用
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