R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SIGIRR (E5M2V) Rabbit mAb #32850
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SIGIRR (E5M2V) Rabbit mAb recognizes endogenous levels of total SIGIRR protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly391 of human SIGIRR protein.
Background
SIGIRR (Single Ig IL-1-related receptor), also known as IL-1R8, belongs to the IL-1 receptor family characterized by the presence of a common intracellular Toll-IL-1 resistance (TIR) domain and extracellular Ig-like domains (1-3). SIGIRR is widely expressed in several epithelial tissues such as liver, lung, and kidney, and in leukocytes including monocytes, T and B lymphocytes, dendritic cells, and natural killer (NK) cells. It negatively modulates the activation upon ligand binding of certain TIR-containing receptors including members of IL-1 receptor family and TLR family (4-6). Recently SIGIRR was identified as a crucial checkpoint receptor in NK cells and a potential therapeutic target in several pathological conditions (7,8).
- Garlanda, C. et al. (2013)
cite>Immunity /cite> 39, 1003-18. - Kang, J.Y. and Lee, J.O. (2011)
cite>Annu Rev Biochem /cite> 80, 917-41. - O'Neill, L.A. (2008)
cite>Immunol Rev /cite> 226, 10-8. - Wald, D. et al. (2003)
cite>Nat Immunol /cite> 4, 920-7. - Anselmo, A. et al. (2016)
cite>Cardiovasc Res /cite> 111, 373-84. - Garlanda, C. et al. (2004)
cite>Proc Natl Acad Sci U S A /cite> 101, 3522-6. - Molgora, M. et al. (2017)
cite>Nature /cite> 551, 110-114. - Molgora, M. et al. (2018)
cite>Scand J Immunol /cite> 88, e12705.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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