Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SH2D2A/TSAd (E2V4X) Rabbit mAb #50147
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 51, 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SH2D2A/TSAd (E2V4X) Rabbit mAb recognizes endogenous levels of total SH2D2A/TSAd protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SH2D2A/TSAd protein.
Background
The T cell-specific adapter protein (TSAd), encoded by the SH2D2A gene, is an adapter molecule containing an SRC homology 2 (SH2) domain and a proline-rich region (PRR) in addition to several tyrosine phosphorylation sites (1). The protein is predominantly expressed in T cells, NK cells, and endothelial cells (2,3). TSAd is required for T cell polarity establishment, interaction with antigen-presenting cells (4), and maintenance of T regulatory cell (Treg) function (5). The protein modulates T cell receptor (TCR) signaling in activated T cells via interaction with the tyrosine kinases Lck (6) and Itk (7). In endothelial cells, TSAd is required for VEGF-induced angiogenic sprouting through its binding to phosphorylated VEGF Receptor 2 (VEGFR2) at Tyr951 and coupling of VEGFR2 signaling to c-Src activation (8,9).
- Spurkland, A. et al. (1998) J Biol Chem 273, 4539-46.
- Nejad, S. et al. (2004) Immunogenetics 56, 338-42.
- Wu, L.W. et al. (2000) J Biol Chem 275, 6059-62.
- Berge, T. et al. (2010) PLoS One 5, e9761.
- Wedel, J. et al. (2019) J Immunol 203, 2328-2338.
- Marti, F. et al. (2006) J Exp Med 203, 281-7.
- Granum, S. et al. (2014) Sci Signal 7, ra118.
- Sun, Z. et al. (2012) J Exp Med 209, 1363-77.
- Matsumoto, T. et al. (2005) EMBO J 24, 2342-53.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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