R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SH2D1B/EAT-2 (E2P5P) Rabbit mAb #58173
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SH2D1B/EAT-2 (E2P5P) Rabbit mAb recognizes endogenous levels of total SH2D1B/EAT-2 protein. This antibody does not cross-react with SH2D1A/SAP protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly92 of human SH2D1B/EAT-2 protein.
Background
SH2 domain-containing protein 1B/EWS/FLI1-activated transcript 2 (SH2D1B/EAT-2) belongs to the SAP family of cytoplasmic signaling adaptor proteins. Structurally, SH2D1B/EAT-2 is highlighted by a single SH2 domain and a short C-terminus containing multiple tyrosine residues whose phosphorylation regulate SH2D1B/EAT-2 function (1,2). Research studies have demonstrated that SH2D1B/EAT-2 is recruited to multiple SLAM family receptors in a phosphotyrosine-dependent manner and that phosphorylation of its C-terminal tyrosine residues is required for control of downstream signaling (3-5). SH2D1B/EAT-2 expression has been detected in multiple hematopoietic cell lineages, including NK cells and macrophages (4,6). Indeed, in murine NK cells, tyrosine phosphorylation of SH2D1B/EAT-2 mediates inhibitory signaling downstream of 2B4/SLAMF4 to suppress effector functions (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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