SH2D1A Antibody #2778
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 14 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SH2D1A Antibody detects endogenous levels of total SH2D1A protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Gly112 of human SH2D1A. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SH2D1A and SH2D1B are small, adaptor proteins with a single SH2-domain that play important signal transduction roles mediated by the signaling lymphocytic activation molecule (SLAM) family receptors (1). SH2D1A (also called SAP or SLAM-associated protein) is frequently mutated in patients with X-linked lymphoproliferative disease (Duncan’s disease), which is characterized by extreme susceptibility to Epstein-Barr virus; approximately 50 different SH2D1A mutations have been reported to date (2-4). The single SH2D1B gene in humans (also called EAT-2 or Ewing's sarcoma's/FLI1-activated transcript 2) is present as a pair of duplicated EAT-2A and EAT-2B genes with identical genomic organization in mouse and rat (5,6).
- Latour, S. and Veillette, A. (2004) Semin. Immunol. 16, 409-419.
- Nichols, K.E. et al. (2005) Immunol. Rev. 203, 180-199.
- Engel, P. et al. (2003) Nat. Rev. Immunol. 3, 813-821.
- Sumegi, J. et al. (2002) Leuk. Lymphoma 43, 1189-1201.
- Roncagalli, R. et al. (2005) Nat. Immunol. 6, 1002-1010.
- Calpe, S. et al. (2006) Immunogenetics 58, 15-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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