SGLT2 Antibody #14210
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46-75 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SGLT2 Antibody recognizes endogenous levels of total SGLT2 protein. This antibody does not recognize SGLT1 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SGLT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Na(+)/glucose cotransporter 2 (SGLT2) is one of the two main glucose transporters in the kidney proximal convoluted tubule. It is activated by Protein Kinase A and Protein Kinase C, likely through phosphorylation of Ser624 (1,2). SGLT2 is responsible for the majority of glucose reabsorption in the kidney (3,4), and mutations in SGLT2 are known to cause familial renal glucosuria (5,6). SGLT2 is a therapeutic target for type 2 diabetes. Inhibitors of SGLT2 have been developed in order to treat people with type 2 diabetes (7).
- Feric, M. et al. (2011) Am J Physiol Cell Physiol 300, C755-70.
- Ghezzi, C. and Wright, E.M. (2012) Am J Physiol Cell Physiol 303, C348-54.
- Wells, R.G. et al. (1992) Am J Physiol 263, F459-65.
- Wright, E.M. (2001) Am J Physiol Renal Physiol 280, F10-8.
- Lee, H. et al. (2012) Pediatr Nephrol 27, 1091-5.
- Andrianesis, V. and Doupis, J. (2013) Expert Rev Clin Pharmacol 6, 519-39.
- Sheridan, C. (2012) Nat Biotechnol 30, 899-900.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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