R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SGK1 (D27C11) Rabbit mAb #12103
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SGK1 (D27C11) Rabbit mAb recognizes endogenous levels of total SGK1 protein. This antibody does not cross-react with SGK2 or SGK3.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human SGK1 protein.
Background
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).
In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9).
In addition to its membrane channel and carrier functions, SGK1 also regulates a broad range of targets, such as GSK-3, NEDD4, iNOS, AMPAR, PSD95, Tau, NF-κB, CREB, and FKHR-L1 (8,9).
- Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
- Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
- Park, J. et al. (1999) EMBO J 18, 3024-33.
- Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
- Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
- Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
- Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.
- Lang, F. et al. (2009) Expert Opin Ther Targets 13, 1303-11.
- Lang, F. et al. (2010) J Physiol 588, 3349-54.
限制使用
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