SF3A3 Antibody #47147
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SF3A3 Antibody recognizes endogenous levels of total SF3A3 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu495 of human SF3A3 protein. Antibodies are purified by peptide affinity chromatography.
Background
The mammalian splicing factor SF3a contains three primary subunits: SF3A1 (SF3a120), SF3A2 (SF3a66), and SF3A3 (SF3a60) which, along with SF3b, activate the U2 snRNP to form the pre-mRNA splicing complex (1,2). SF3A2 and SF3A3 both interact with SF3A1, but not with each other, and SF3A3 plays a major role in recruiting SF3A1 to the complex (3). Each protein in the heterotrimer has been shown to be essential for mRNA splicing and cell viability (4). Notably, SF3a plays a role in growth factor signaling as SF3A1 can bind to ERalpha when phosphorylated at Ser118 to affect downstream splicing (5). SF3a also plays a role in the innate immune response, as it promotes inflammation by splicing the TLR adaptor Myd88. Furthermore, SF3a regulates a number of genes involved in innate immune signaling pathways in macrophages versus other pathways (6,7).
- Brosi, R. et al. (1993) Science 262, 102-5.
- Brosi, R. et al. (1993) J Biol Chem 268, 17640-6.
- Nesic, D. and Krämer, A. (2001) Mol Cell Biol 21, 6406-17.
- Tanackovic, G. and Krämer, A. (2005) Mol Biol Cell 16, 1366-77.
- Masuhiro, Y. et al. (2005) Proc Natl Acad Sci U S A 102, 8126-31.
- De Arras, L. and Alper, S. (2013) PLoS Genet 9, e1003855.
- O'Connor, B.P. et al. (2015) PLoS Genet 11, e1004932.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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