SET1A (D3V9S) Rabbit mAb #61702

Filter:

WB
IP
ChIP
C&R
C&T

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	300
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
ChIP-Chromatin Immunoprecipitation
C&R-CUT & RUN
C&T-CUT & Tag

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Chromatin IP	1:100
Chromatin IP-seq	1:100
CUT&RUN	1:100
CUT&Tag	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SET1A (D3V9S) Rabbit mAb recognizes endogenous levels of total SET1A protein. This antibody does not cross-react with other SET1/COMPASS proteins, including SET1B, MLL1, MLL2, MLL3, and MLL4.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro383 of human SET1A protein.

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).

Database Links

UniProt ID: O15047

Entrez-Gene Id: 9739

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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