R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Sec61A1 (D7Q6V) Rabbit mAb #14868
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Sec61A1 (D7Q6V) Rabbit mAb recognizes endogenous levels of total Sec61A1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg273 of human Sec61A1 protein.
Background
Sec61 translocon is a channel complex located on the endoplasmic reticulum (ER) membrane to mediate membrane protein insertion into the organelle (1). There are three components in the complex, Sec61A, Sec61B, and Sec61G (2). Sec61A is the main component of the channel on the ER membrane and directly contacts nascent synthesized polypeptide TMD (transmembrane domain) for insertion (3). Sec61G functions in stablizing the channel (3). In addition to TMD insertion, Sec61 translocon has also been shown to be involved in ER calcium leakage (4,5). Both Bip and calmodulin can inhibit this leakage by their interaction with Sec61A (6,7). Sec61B has no obvious function related to target protein ER membrane insertion, but is involved in other vesicle trafficking processes such as EGFR and Her2 trafficking from the cytosol to nucleus (8,9), Gurken trafficking from Golgi to plasma membrane (10), and copper-transporting ATPase membrane distribution (11).
- Shao, S. and Hegde, R.S. (2011) Annu Rev Cell Dev Biol 27, 25-56.
- Hartmann, E. et al. (1994) Nature 367, 654-7.
- Van den Berg, B. et al. (2004) Nature 427, 36-44.
- Flourakis, M. et al. (2006) FASEB J 20, 1215-7.
- Lang, S. et al. (2001) Channels (Austin) 5, 228-35.
- Erdmann, F. et al. (2011) EMBO J 30, 17-31.
- Schäuble, N. et al. (2012) EMBO J 31, 3282-96.
- Wang, Y.N. et al. (2010) J Biol Chem 285, 38720-9.
- Wang, Y.N. et al. (2012) J Biol Chem 287, 16869-79.
- Kelkar, A. and Dobberstein, B. (2009) BMC Cell Biol 10, 11.
- Abada, P.B. et al. (2012) Mol Pharmacol 82, 510-20.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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