Sec23A Antibody #8162
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 82 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Sec23A Antibody recognizes endogenous levels of total Sec23A protein. Based on sequence homology, this antibody may cross-react with Sec23B.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe717 of human Sec23A protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
There are two isoforms of Sec23 protein: Sec23A and Sec23B. Both isoforms have been shown in the Sec23/24 complex, which is a component of COPII coat (1). COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (2). COPII formation is initiated through binding of the activated G protein, Sar1, to the Sec23/24 complex to form a prebudding complex, which directly binds target molecules (2-4). The prebudding complex further recruits Sec13/31 to form mature COPII coat (5,6). In addition to being a COPII component, Sec23 has also been shown to interact with p125 and Sec16 at the transitional ER; these interactions are important for regulation of the COPII transportation function (7,8).
- Paccaud, J.P. et al. (1996) Mol Biol Cell 7, 1535-46.
- Aridor, M. et al. (1998) J Cell Biol 141, 61-70.
- Miller, E.A. et al. (2003) Cell 114, 497-509.
- Mossessova, E. et al. (2003) Cell 114, 483-95.
- Barlowe, C. et al. (1994) Cell 77, 895-907.
- Bi, X. et al. (2007) Dev Cell 13, 635-45.
- Iinuma, T. et al. (2007) J Biol Chem 282, 17632-9.
- Hughes, H. et al. (2009) J Cell Sci 122, 2924-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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